Customized Library Preps for NGS
- custom DNA-seq or RNA-seq
- Amplification of cDNA or gDNA using SMART-Seq kit (TaKaRa Bio) or REPLI-g (for cDNA or DNA from Qiagen)
We recommend collecting your cells or tissue in Trizol (ThermoFisher) or DNA/RNA shield (Zymo). Please contact us first if you have less than 100 cells, or you have a degraded RNA sample.
- miRNA-seq/Small RNA-seq
We are using RealSeq (Somagenics) to construt miRNA-seq libraries from total RNA (100ng). The rate is same as RNA-seq Low-input plus Pippin prep.
- Pippin prep
We are using Pippin prep to size select for NGS-libraries, and this service can be used as a customized service. Please contact us if you are interested in using the Pippin prep service.
- Captured library prep – Exome, custom panels…
We can provide this service as a collaboration project, or a trial project if you do not have any experience with Molecular Biology methods or cannot access a molecular biology lab.
Please contact us if you do not find your application in the services, provided by QB3 Genomics team. We might be able to help you as a customized collaboration project.